The invisible anti-CDV antibody(T line) and secondary antibody (C line) are immo-bilized on a nitrocellulose membrane in result window.

Another anti-CDV antibody isconjugated with colloidal gold particle and immobilized on glass fiber as conjugatepad.When specimen is applied into the sample well on device,CDV antigen will bindto the gold conjugate and migrate on the surface of test strip by passive diffusion.

and a visible T line will appear in result window after 10 minutes.The C line has toappear every time when the test has performed which indicates the test is performingproperly